HPLC 2016

44th International Symposium on High Performance Liquid Phase Separations and Related Techniques

June 19 – 24, 2016 | Marriott San Francisco Marquis, San Francisco, California

Join us in Booth 601

From sample preparation to final results, discover how innovative Thermo Scientific™ chromatography instruments, combined with columns and consumables, software and mass spectrometry technology help you meet your analytical challenges. We’ll also be featuring solutions for pharmaceutical and biopharmaceutical workflows.

Join our Workshops featuring scientific guest speakers:

Tuesday, June 21 | 12:20 – 1:20 p.m.

Unlimited Possibilities: Using Next Generation UHPLC and Column Chemistries to Cross Chromatographic Boundaries

Discover how the separation and detection capabilities of the Thermo Scientific™ Vanquish™ UHPLC platform deliver unsurpassed results. System performance will be demonstrated by a distinguished research team at the University of Michigan who explore the performance limits of UHPLC with various column formats, and who use Vanquish as a routine UHPLC together with the high resolution MS in their applied research.

Speakers: Dr Frank Steiner, Thermo Fisher Scientific, Germany; Dr. James Grinias, University of Michigan

Wednesday, June 22 | 12:20 – 1:20 p.m.

Unlocking Biomolecular Secrets: Comprehensive Biopharmaceutical Characterization Workflows

We will discuss a number of tools used to help in advancing research in aggregate, glycan, charge variant, peptide sequence and intact mass analysis of monoclonal antibodies. You will also get firsthand insights on how the Christian Doppler Laboratory for Biosimilar Characterization develops these workflows further to uncover a more in depth understanding of protein chromatography and protein column characterization.

Speakers: Dr Rowan Moore, Thermo Fisher Scientific, UK; Prof Christian G Huber, Paris Lodron University Salzburg, Austria

Ion Chromatography Tutorial

June 23 | 1:15 – 2:30 p.m.
Yerba Buena Ballroom Salons 5/6, floor lower B2 level

High Pressure Ion Chromatography: Past, Present and Future
This tutorial will cover the history of High Pressure Ion Chromatography (HPIC), review the current state-of-the-art and look at possible future developments. HPLC and HPIC have seen a steady trend toward smaller particle size and shorter columns. This tutorial will review the reasons behind the slower rate of change for HPIC and describe the factors necessary to achieve parity with HPLC with respect to particle size. It will also review the range of HPIC materials currently available, provide application examples and look at possible progress areas.

Oral Presentations

June 21 | 11:15 - 11:35 a.m. | Session 3B: Biopharmaceutical Analysis –II
High Resolution LC/MS analysis of Therapeutic Oligonucleotides on a New Porous Polymer-Based Reversed Phase Column
Location: Golden Gate Ballroom B, floor B2 level

June 22 | 1:55 - 2:20 p.m. | Session 10A: Stationary Phases
Synthesis and Applications of pH Reversible Ion Exchange Materials
Location: Golden Gate Ballroom A, floor B2 level

June 22 | 4:15 - 4:40 p.m. | Session 6B: Biohpharmaceutical Analysis - III
What HPLC-HRMS Can Tell Us About Posttranslational Modifications in Therapeutic Proteins: Focus on Oxidation and Glycosylation
Location: Golden Gate Ballroom B, floor B2 level

June 23 | 9:20 - 9:40 a.m. | Session 12B: Challenges for Pharma - Method Development and Evaluation
Role of Structural Similarity in Prediction of Retention in Reversed-Phase, IonExchange and Hydrophilic Interaction Liquid Chromatography Modes Using Quantitative Structure-Retention Relationships
Location: Golden Gate Ballroom B, floor B2 level

June 24 | 2:20 - 2:40 p.m. | Session 19C: Ion Chromatography
On the Development and Assessment of Microfluidic Membrane Suppressors Featuring Integrated On-chip Conductivity Detection for Capillary Ion-Exchange Chromatography
Location: Golden Gate Ballroom C, floor B2 level

June 24 | 2:40 - 3:00 p.m. | Session 19C: Ion Chromatography
Determination of Small Polar Metabolites using Column Switching with Ion Chromatography and High Resolution Accurate Mass Spectroscopy
Location: Golden Gate Ballroom C, floor B2 level

Poster Presentations

Screening of Phosphodiesterase Type 5 Inhibitors in Dietary Supplements using Liquid Chromatography/Quadrupole–orbital Ion Trap Mass Spectrometry

Analysis of N-glycans Derived from Glycoproteins - HPAE-PAD versus Mixed-mode Liquid Chromatography

A Quality-by-Design Methodology for Rapid HILIC Method Development in Pharmaceutical Analysis

Retention Prediction of Inorganic Anions and Organic Cations in Ion Chromatography based on Quantitative Structure-retention Relationships

Middle-down Approach for Monitoring Monoclonal Antibody Variants and Deglycosylation

Separating Eluent and Column Thermostatting for Best Possible Selectivity and Efficiency in Critical UHPLC Methods

Determination of Inorganic Cations and Ammonium in Environmental Waters using a Compact Integrated High-pressure Ion Chromatography System

Faster and Improved Ease-of-use Assays of Citrate and Phosphate in Pharmaceutical Formulations using Ion Chromatography with Suppressed Conductivity

Fast Sugar Analysis for Food and Beverage using HPAE-PAD on a Compact IC System

Capillary Electrophoresis – Mass Spectrometry for Top Down Proteomics

A Comprehensive Study to Protein Retention in Hydrophobic Interaction Chromatography

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Possibilities and Limitations of Microfluidic Membrane Suppressors for Capillary Anion-exchange Chromatography Hyphenated with On-chip Conductivity Detection

Guidelines for Method Transfer and Optimization of the Corona Veo Charged Aerosol Detector

Direct Measurement of Sialic Acids Released from Glycoproteins, by High Performance Liquid Chromatography and Charged Aerosol Detection

Characterization of a Biologic Therapeutic: Reversed Phase Analysis of Protein and Excipients

Characterization and Lot-to-lot Variability of Complex Surfactants by High Performance Liquid Chromatography and Charged Aerosol Detection

A New Silica Particle Designed for the Separation of Large Molecules

HPAE-FLD Method for Separation of Human Alpha1 Acid-Glycoprotein and Bovine Fetuin 2- Aminobenzamide (2-AB)-labeled Oligosaccharides

Label-free Characterization of O-linked Glycans by HPLC with Charged Aerosol Detection

Directly Monitor Peptide PEGylation by HPLC with Charged Aerosol Detection

A Fast Method for Sugar Analysis of Instant Coffee Samples

Determination of Pyrethrins in Pyrethrum Oil Extracts

Site Specific Characterization of Cetuximab N-Glycosylation using Multidimensional Liquid Chromatography